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optimal filter sets  (Nikon)


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    Nikon optimal filter sets
    Optimal Filter Sets, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 57094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optimal+filter+sets/pmc13007337-184-24-28?v=Nikon
    Average 99 stars, based on 57094 article reviews
    optimal filter sets - by Bioz Stars, 2026-06
    99/100 stars

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    A. fluorescent proteins, without fused sequences, were continuously irradiated for 300 seconds with appropriate filter sets, with images captured every two seconds in live NIH 3T3 cells. Monochrome images were quantified using Image J (NIH), normalized to start fluorescent levels, and plotted over time. Deviations across three experiments for each fluorophore was <5%. B. PSCFP2 does not photoactivate with 175W unfiltered xenon light over 15 minutes constant irradiation. Light was provided without filtration through a liquid light guide and using a plan apochromat NA1.3 63X oil immersion objective (Nikon). While slight photobleaching was noted, (panels a versus c), no cyan fluorescence was detected above CCD camera noise (panels b,d). Scale bar is 10 μm.

    Journal: Biological Procedures Online

    Article Title: Practical three color live cell imaging by widefield microscopy

    doi: 10.1251/bpo119

    Figure Lengend Snippet: A. fluorescent proteins, without fused sequences, were continuously irradiated for 300 seconds with appropriate filter sets, with images captured every two seconds in live NIH 3T3 cells. Monochrome images were quantified using Image J (NIH), normalized to start fluorescent levels, and plotted over time. Deviations across three experiments for each fluorophore was <5%. B. PSCFP2 does not photoactivate with 175W unfiltered xenon light over 15 minutes constant irradiation. Light was provided without filtration through a liquid light guide and using a plan apochromat NA1.3 63X oil immersion objective (Nikon). While slight photobleaching was noted, (panels a versus c), no cyan fluorescence was detected above CCD camera noise (panels b,d). Scale bar is 10 μm.

    Article Snippet: NIH 3T3 cells expressing 500ng of transiently transfected fluorophore-producing plasmids were imaged at 24 hours post transfection using filter sets optimized for each fluorophore (all Semrock sets).

    Techniques: Irradiation, Filtration, Fluorescence